Establishment of a Novel Anti-CD44 Variant 10 Monoclonal Antibody C44Mab-18 for Immunohistochemical Analysis against Oral Squamous Cell Carcinomas
نویسندگان
چکیده
Head and neck squamous cell carcinoma (HNSCC) is the most common type of head cancer, has been revealed as second-highest expression CD44 in cancers. investigated a cancer stem marker HNSCC plays critical role tumor malignant progression. Especially, splicing variant isoforms (CD44v) are overexpressed cancers considered promising target for diagnosis therapy. We developed monoclonal antibodies (mAbs) against by immunizing mice with CD44v3–10-overexpressed PANC-1 cells. Among established clones, C44Mab-18 (IgM, kappa) reacted CHO/CD44v3–10, but not CHO/CD44s parental CHO-K1 using flow cytometry. The epitope mapping peptides that cover exon-encoded regions recognized border sequence between 10 constant exon 16-encoded sequence. These results suggest recognizes 10-containing CD44v, CD44s. Furthermore, could recognize human oral (OSCC) line, HSC-3, apparent dissociation (KD) CHO/CD44v3–10 HSC-3 was 1.6 × 10−7 M 1.7 M, respectively. detected CD44v3–10 Western blotting, endogenous CD44v10 immunohistochemistry OSCC tissues. indicate useful detecting cytometry immunohistochemistry.
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ژورنال
عنوان ژورنال: Current Issues in Molecular Biology
سال: 2023
ISSN: ['1467-3037', '1467-3045']
DOI: https://doi.org/10.3390/cimb45070333